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. Author manuscript; available in PMC: 2020 Nov 2.
Published in final edited form as: Methods Mol Biol. 2013;971:295–312. doi: 10.1007/978-1-62703-269-8_17

Table 3.

Run performance metrics

Matrix Description Normal value range
Cluster Density (1,000/mm2) Density of clusters detected by image analysis 200–820 K/mm2
Cluster PF (%) Percentage of clusters passing intensity filter criteria 60–90%
Phasing/Prephasing Value used by RTA for the percentage of molecules in a cluster for which sequencing falls behind (phasing) or jumps ahead (prephasing) the current cycle within a read Phasing < Prephasing Phasing <0.5 Prephasing <0.7
Q30+ (%) Percentage of bases with a Phred score of 30 or higher ≥80%
Mismatch Error Rate The calculated error rate, as determined by the alignment of control (e.g., Phix) <2%