Figure 3.

Optogenetic control of LANS-Set2 reveals genome-wide H3K36 methylation and demethylation kinetics. (A) Genome browser ChIP-seq signal track of a representative example of LANS-Set2 activation (green) and inactivation (purple) over the time course experiment. Signal is normalized by H3 ChIP-seq signal and scaled by the internal spike-in S. pombe DNA. (B,C) Distribution of mean, per-gene normalized LANS-Set2 activation (B) or LANS-Set2 inactivation (C). ChIP-seq signal represented as interquartile range box plots over the time course. Each line represents the mean of the replicates for a specific gene over time. (D,E) Distribution of the relative LANS-Set2 activation (D) or LANS-Set2 inactivation (E) ChIP-seq signal over time. To highlight relative H3K36me3 changes for each gene, the maximum signal value over all time points was set to 1, and subsequent time points became a fraction of that maximum.