Figure 2.

Estimation of RNA synthesis and degradation rates of individual genes. (A) Kinetics model of gene expression. In this model, a transcript was synthesized according to the RNA synthesis rate, k_s (CPM/min) and degraded according to the degradation rate, k_d (min⁻¹). (B) Synthesis rates were estimated by fitting of the time series of the amount of newly synthesized RNAs from individual genes to a logarithmically increasing curve (see Methods). β₁ and t₀ indicate the basal value of newly synthesized RNAs and the time delay, respectively. (C) Degradation rates were estimated by fitting of the time series of the amount of pre-existing RNAs from individual genes to an exponentially decreasing curve (see Methods). β₂ indicates the basal value of pre-existing RNAs. (D,E) Distribution of estimated RNA synthesis rate (D) and degradation rate (E). (F,G) Gene Set Enrichment Analysis (GSEA) for genes with k_s values (F) or with k_d values (G).