Fig. 5.

FSS-induced ATP-dependent Ca²⁺ oscillations regulate osteocyte gene expression. Ocy454 were treated with vehicle (control) or apyrase (10 U/ml) and subjected to static culture conditions (No Flow) or 4 dynes/cm² FSS (Flow) for 5 min. Four to six hours post flow, RNA was isolated, reverse transcribed and gene expression was quantified by real time quantitative PCR. Average raw CT values are available in Supplemental Table I. Graphs depict mean ± SEM from n = 6 independent samples. *p < 0.05, **p < 0.01 compared to the corresponding no flow control. n.s., not significant.