Table 1.
Cycle threshold for values of five primer sets for each experimental condition. RNase P is used as an indicator in clinical specimens that sufficient human cellular material was collected, as well as an extraction/procedural control, and was included as reference. RNase P was likely present on N95 masks from skin contact during handling
| Sample | E-gene (Ct) | N-gene (Ct) | CDC N1 (Ct) | CDC N2 (Ct) | CDC N3 (Ct) | RNase P (Extraction Control) (Ct) |
|---|---|---|---|---|---|---|
| SARS-CoV-2 initial RNA | ||||||
| REF | 20.67 | 26.67 | 19.1 | 18.45 | 19.33 | 32.1 |
| ETOH 1860 | 26.23 | 32.63 | 25 | 28.1 | 26.1 | 33.2 |
| ETOH 1870+ | 30.33 | 37.01 | 28.4 | 33.1 | 30.4 | 33.5 |
| ETOH 8511 | 26.9 | 32.62 | 25.2 | 28.5 | 26.5 | 33.3 |
| + Cntrl 1860 | 21.16 | 27.28 | 19.5 | 19.9 | 19.7 | 31.3 |
| + Cntrl1870+ | 21.43 | 27.19 | 19.4 | 19.9 | 19.6 | 30.9 |
| + Cntrl 8511 | 20.76 | 26.72 | 19 | 19.4 | 19.4 | 32.5 |
| UV 1860 | 20.95 | 27.14 | 19.2 | 19.9 | 19.8 | 33.2 |
| UV Cntrl 1870+ | 23.54 | 29.78 | 21.2 | 22.1 | 21.6 | 34.7 |
| UV Cntrl 8511 | 21 | 27.3 | 19.3 | 19.9 | 19.5 | 34.3 |
| VHP 1860 | 21.93 | 28.57 | 20.2 | 20.6 | 20.6 | 32.8 |
| VHP 1870+ | 22.13 | 28.68 | 20.4 | 21 | 20.8 | 31.6 |
| VHP 8511 | 20.66 | 27.01 | 19 | 19.5 | 19.3 | 31.5 |
| - Cntrl | Undetected | Undetected | Undetected | Undetected | Undetected | 36.1 |
| SARS-CoV-2 cell culture infectivity RNA | ||||||
| REF | 11.85 | 15.96 | 10.7 | 11 | 11.1 | 32.9 |
| ETOH 1860 | Undetected | Undetected | Undetected | Undetected | Undetected | 43.3 |
| ETOH 1870+ | Undetected | Undetected | Undetected | Undetected | Undetected | 41.7 |
| ETOH 8511 | Undetected | Undetected | Undetected | Undetected | Undetected | 40.1 |
| + Cntrl 1860 | 11.96 | 15.72 | 10.8 | 11.1 | 11.2 | 34.4 |
| + Cntrl 1870 | 11.87 | 15.8 | 10.7 | 11.1 | 11.2 | 34.3 |
| + Cntrl 8511 | 12.02 | 16.32 | 10.9 | 11.2 | 11.3 | 35 |
| UV 1860 | 11.76 | 15.83 | 10.6 | 11 | 11.1 | 35.7 |
| UV Cntrl 1870+ | 28.73 | 34.04 | 25.1 | 26.6 | 26.8 | 38.6 |
| UV Cntrl 8511 | 11.83 | 15.88 | 10.7 | 11.1 | 11 | 33.6 |
| VHP 1860 | 27.33 | 33.22 | 24.6 | 26.2 | 26.1 | 39 |
| VHP 1870+ | 28.79 | 33.23 | 25.3 | 25.9 | 25.9 | 38.3 |
| VHP 8511 | 11.66 | 15.75 | 10.7 | 11.1 | 11.1 | 33.6 |
| - Cntrl | Undetected | Undetected | 36.5 | 35.1 | Undetected | 34.5 |