Skip to main content
. 2020 Oct 28;40(44):8438–8462. doi: 10.1523/JNEUROSCI.1091-20.2020

Figure 7.

Figure 7.

The PTPσ Ig domain is necessary and sufficient for interaction with Nrxn1α. A, Schematic diagrams of PTPσ WT and deletion mutants. B, Representative images of cell-surface binding assays. HEK293T cells expressing HA-Nlgn1, HA-PTPσ WT, HA-PTPσ ΔIg, or the indicated PTPσ Ig domain splicing variants constructs were incubated with 10 μg/ml of control IgC or Ig-Nrxn1α-SS4 and then analyzed by immunofluorescence imaging of Ig-fusion proteins (red) and HA antibodies (green). Scale bar, 10 μm. C, Quantification of the average red intensities in the green positive region of HEK293T cells in B. n indicates the number of cells as follows: Ig-Nrxn1α/Nlgn1, n = 27; Ig-Nrxn1α/PTPσ-full, n = 33; Ig-Nrxn1α/PTPσ ΔIg, n = 32; Ig-Nrxn1α/PTPσ IgA–B–, n = 30; Ig-Nrxn1α/PTPσ IgA+B–, n = 22; Ig-Nrxn1α/PTPσ IgA–B+, n = 27; Ig-Nrxn1α/PTPσ IgA+B+, n = 23; IgC/Nlgn1, n = 10; IgC/PTPσ-full, n = 11; IgC/PTPσ ΔIg, n = 15; IgC/PTPσ IgA–B–, n = 11; IgC/PTPσ IgA+B–, n = 11; IgC/PTPσ IgA–B+, n = 12; and IgC/PTPσ IgA+B+, n = 10.