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. 2020 Oct 28;40(44):8438–8462. doi: 10.1523/JNEUROSCI.1091-20.2020

Table 1.

Lentiviral shRNA constructs used in the current study

shRNA construct Target nucleotide sequence (5′−3′) Reference KD efficiency (%)
L-309 sh-PTPσ GCCACACACCTTCTATAAT Yim et al., 2013 89.7 ± 3.2
L-309 sh-PTPδ GTGCCGGCTAGAAACTTG Yim et al., 2013 93.5 ± 1.6
L-315 sh-β-catenin GCAATCAGCTGGCCTGGTTTG Han et al., 2018 83.8 ± 8.0
L-315-sh-Abl1/2 AACCTGTACACATTCTGTGTG Current study 81.4 ± 0.7
L-315-sh-p250RhoGAP ACAAGAAGCACCAAGTA Takamori et al., 2000 38.3 ± 5.9
L-315-sh-Ena TTGTGGAAGAGGTGCGGAA Current study 71.8 ± 4.0
L-315-sh-MIM-B CCGGTTCTGCACCTTCATT Current study 85.6 ± 6.4
L-315-sh-CASK GCTGAAGCATCCACACATTGT Current study 78.2 ± 4.1
L-315-sh-Caskin-1 GGAGATCAAGCAACGGCTTCG Current study 90.6 ± 3.1
L-315-sh-Caskin-2 GCTGATCTCAGGCCAGATTTC Current study 51.3 ± 7.1
L-315-sh-RIM1 AGTCCACAGGGTAAAGTTC Spangler et al., 2013 47.4 ± 7.8
L-315-sh-RIM-BP1 GCCAGATCCTCAAGGTGTTTG Current study 64.2 ± 9.8
L-315-sh-RIM-BP2 GGAGCAAATGTCACAGGATAT Current study 49.7 ± 9.7
L-315 sh-Liprin-α2 AGCCAGTCTGATTACAGAA Spangler et al., 2013 74.9 ± 7.6
L-315 sh-Liprin-α3 GCTAACATGAAGAAGCTTCAA Han et al., 2018 82.1 ± 5.8
L-315-sh-Piccolo AAGTGCTGTCTCCTCTGTTGT Spangler et al., 2013 78.8 ± 11.9
L-315-sh-ELKS1 GCTCGGGATGAGTCCATTAAG Current study 66.3 ± 2.4
L-315-sh-ELKS2 GCAAGGAGCTTGACATTAAGG Current study 87.6 ± 2.9
L-315-sh-Trio GCAGTTCCAGCACGCTATTG Current study 70.1 ± 3.7
L-315-sh-SYD1A GCAGCTTCTGGTAGAACGTGA Current study 50.9 ± 8.6
L-315-sh-N-cadherin GGACAACTGTCAGTCACAAAG Han et al., 2018 70.3 ± 5.9
L-315-Nrxn TKD GTGCCTTCCTCTATGACAACT (for Nrxn1); GAACAAAGACAAAGAGTAT (for Nrxn2); and ATGCTACACTTCAGGTGGACA (for Nrxn3) Um et al., 2014 86.7 ± 3.6