Table 1.
shRNA construct | Target nucleotide sequence (5′−3′) | Reference | KD efficiency (%) |
---|---|---|---|
L-309 sh-PTPσ | GCCACACACCTTCTATAAT | Yim et al., 2013 | 89.7 ± 3.2 |
L-309 sh-PTPδ | GTGCCGGCTAGAAACTTG | Yim et al., 2013 | 93.5 ± 1.6 |
L-315 sh-β-catenin | GCAATCAGCTGGCCTGGTTTG | Han et al., 2018 | 83.8 ± 8.0 |
L-315-sh-Abl1/2 | AACCTGTACACATTCTGTGTG | Current study | 81.4 ± 0.7 |
L-315-sh-p250RhoGAP | ACAAGAAGCACCAAGTA | Takamori et al., 2000 | 38.3 ± 5.9 |
L-315-sh-Ena | TTGTGGAAGAGGTGCGGAA | Current study | 71.8 ± 4.0 |
L-315-sh-MIM-B | CCGGTTCTGCACCTTCATT | Current study | 85.6 ± 6.4 |
L-315-sh-CASK | GCTGAAGCATCCACACATTGT | Current study | 78.2 ± 4.1 |
L-315-sh-Caskin-1 | GGAGATCAAGCAACGGCTTCG | Current study | 90.6 ± 3.1 |
L-315-sh-Caskin-2 | GCTGATCTCAGGCCAGATTTC | Current study | 51.3 ± 7.1 |
L-315-sh-RIM1 | AGTCCACAGGGTAAAGTTC | Spangler et al., 2013 | 47.4 ± 7.8 |
L-315-sh-RIM-BP1 | GCCAGATCCTCAAGGTGTTTG | Current study | 64.2 ± 9.8 |
L-315-sh-RIM-BP2 | GGAGCAAATGTCACAGGATAT | Current study | 49.7 ± 9.7 |
L-315 sh-Liprin-α2 | AGCCAGTCTGATTACAGAA | Spangler et al., 2013 | 74.9 ± 7.6 |
L-315 sh-Liprin-α3 | GCTAACATGAAGAAGCTTCAA | Han et al., 2018 | 82.1 ± 5.8 |
L-315-sh-Piccolo | AAGTGCTGTCTCCTCTGTTGT | Spangler et al., 2013 | 78.8 ± 11.9 |
L-315-sh-ELKS1 | GCTCGGGATGAGTCCATTAAG | Current study | 66.3 ± 2.4 |
L-315-sh-ELKS2 | GCAAGGAGCTTGACATTAAGG | Current study | 87.6 ± 2.9 |
L-315-sh-Trio | GCAGTTCCAGCACGCTATTG | Current study | 70.1 ± 3.7 |
L-315-sh-SYD1A | GCAGCTTCTGGTAGAACGTGA | Current study | 50.9 ± 8.6 |
L-315-sh-N-cadherin | GGACAACTGTCAGTCACAAAG | Han et al., 2018 | 70.3 ± 5.9 |
L-315-Nrxn TKD | GTGCCTTCCTCTATGACAACT (for Nrxn1); GAACAAAGACAAAGAGTAT (for Nrxn2); and ATGCTACACTTCAGGTGGACA (for Nrxn3) | Um et al., 2014 | 86.7 ± 3.6 |