Table 3.
Oligonucleotides for rescue vectors used in the current study
| Gene | Species | Oligo sequence (5′−3′) |
|---|---|---|
| Rimbp2 | Rat |
For mutagenesis Forward: CGTACACGCCATATCCTGTTACGTTGGCACCATTGGACAACCCAGTGG Reverse: CCACTGGGTTGTCCAATGGTGCCAACGTAACAGGATATGGCGTGTACG |
|
For L-313 vector cloning Forward: TCCCGAATTCGCTAGCGCCACCATGCGAGAGGCTGCT Reverse: CCGCTTTACTTGTACATTAGGGTGTGAAATGAACACT | ||
| Caskin-1 | Rat |
For first mutagenesis Forward: AGGCCAGCAAGGAAATTAAGCAACTGCTTCGAGAGGCT Reverse: AGCCTCTCGAAGCAGTTGCTTAATTTCCTTGCTGGCCT |
|
For second mutagenesis Forward: CAGCAAGGAAATTAAGCAACTGCTCCGAGAGGCT Reverse: AGCCTCTCGGAGCAGTTGCTTAATTTCCTTGCTG | ||
|
For L-313 vector cloning Forward:TCCCGAATTCGCTAGCGCCACCATGGGGAAGGAGCAGG Reverse:CCGCTTTACTTGTACATCACTCCAGCATGGC | ||
| Syd1a | Mouse |
For mutagenesis Forward: GGGACTGCTCACGCTCCACCAGGAGTTGCAGAGGGAGCCCG Reverse: CGGGCTCCCTCTGCAACTCCTGGTGGAGCGTGAGCAGTCCC |
|
For L-313 vector cloning (including HA tag) Forward: TCCCGAATTCGCTAGCGCCACCATGTACCCCTACGACG Reverse: CCGCTTTACTTGTACATCAGAGGCACACATTGATC | ||
| Ena | Human |
For L-313 vector cloning Forward: TCCCGAATTCGCTAGCGCCACCATGAGTGAACAGAGTATCTGTCA Reverse: CCGCTTTACTTGTACACTATGCAGTATTTGACTTGCTC |
| Abl1 | Human |
For L-313 vector cloning (including HA tag) Forward: TCCCGAATTCGCTAGCGCCACCATGTATCCTTACGACGTGCC Reverse: CCGCTTTACTTGTACATTACCTCTGCACTATGTCACT |
| Mim-b | Human |
For L-313 vector cloning Forward: TCCCGAATTCGCTAGCGCCACCATGGAGGCTGTGATTGAGAA Reverse: CCGCTTTACTTGTACA CTAAGAAAAGCGAGGGGC |
| Cask | Rat |
For mutagenesis Forward: GCGGAATTCGCCACCATGGAGACAGACACACTCC Reverse: CGCGTCGACGGTGATTGGGTCCAAAGTTG |
|
For L-313 vector cloning Forward: TCCCGAATTCGCTAGCGCCACCATGGCCGACGACGA Reverse: CCGCTTTACTTGTACACTAATAGACCCAGGAGACCG |