Figure 3.

Expression of transcripts for NADPH oxidase subunits and other antioxidant enzymes, and increased lipid peroxidation, in middle-aged CNS white matter lesions. A, Raw FPKM (fragments per kilobase of exon model per million reads mapped) levels of NADPH oxidase subunits. Cybb and Ncf2 are more highly expressed in middle-aged lesions. B, Representative micrographs of coronal spinal cord sections stained with antibodies against MDA and MBP. There is noticeable spatial overlap between the two stains. Scale bar, 25 μm. C, Quantification of MDA in middle-aged and young animals suggests oxidative stress is greater in middle-aged animals (p = 0.0002). D, Heatmap of endogenous antioxidant enzyme expression. All animals are on the C57BL/6 wild-type strain.