Figure 1.

Transgenic approach to study auditory cortex Foxp2+ neurons in mice carrying the R552H mutation. A, Double immunostaining for EGFP (in green) and Foxp2 (in red) in a coronal brain slice from an Ntsr1-EGFP mouse. Scale bar, 500 µm. B, Enlargement of the boxed area in A, showing the expression of Foxp2 predominantly in Layer VI in auditory cortex (dashed white lines delineate cortical layers). Scale bar, 250 µm. C, Layer VI EGFP+ neurons in Ntsr1-EGFP mice also express Foxp2. Scale bar, 20 µm. D, Quantification of EGFP-Foxp2 colocalization in Layer VI (n = 7 coronal sections from 3 mice); histograms represent the mean ± SEM; full circles show individual sections.