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. 2020 Sep 25;39(44):6856–6870. doi: 10.1038/s41388-020-01465-y

Fig. 3. ERBB signaling in SCaBER and J82 bladder cancer cells upon TKI treatment and EGF stimulation.

Fig. 3

a Western blot analyses illustrate activation and inhibition of EGFR/p-EGFR (Tyr1068, Tyr1045), ERK/p-ERK (Thr202,Tyr204), and p-AKT (Ser473) 24 h after EGF and erlotinib treatment. DMSO application was used as untreated control. β-actin (for EGFR) and tubulin (for ERK) served as loading controls. b–i Relative mRNA expression of ERBB receptors (EGFR, ERBB2, ERBB3, and ERBB4), ERBB ligands (AREG, EREG, and HB-EGF) and the EGFR target gene SOX9 normalized to corresponding DMSO control 24 h after EGF and/or erlotinib treatment for SCaBER and J82 bladder cancer cells. GAPDH was used for standardization. FC: fold change. Vertical lines: +standard error of mean (SEM) of triplicates.