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. Author manuscript; available in PMC: 2020 Nov 2.
Published in final edited form as: ACS Chem Neurosci. 2020 Apr 2;11(8):1178–1191. doi: 10.1021/acschemneuro.0c00069

Figure 5.

Figure 5.

The effect of fresh Aβ42 peptides and their aggregates on SH-SY5Y neuroblastoma cell health was measured by (A) MTS assay, (B) DCFH-DA assay, and (C) LDH assay. The fresh peptides, 72 h, or 7 d aggregates were added to the cells to a final concentration of 5 μM. Cells were incubated for 48 h in the presence of peptides for MTS and LDH assay and for 24 h for DCFH-DA assay. For the t-BHP control for the DCFH-DA assay, cells were incubated with 50 μM t-BHP for 4 h at 37 °C. The mixtures represent samples containing equimolar (1:1) WT and acetylated peptides. Error bars = ±SD (for A n = 6; for B n = 5; and for C n = 5). *, **, and *** represent significant differences (* = p < 0.05), (** = p < 0.005), and (*** = p < 0.0005) among 7 d Aβ42 aggregated peptides.