Figure 6.

The effect of fresh Aβ42 peptides and their aggregates on primary neuronal cell health was measured by (A) DCFH-DA assay and (B) LDH assay. The fresh peptides, 72 h, or 7 d aggregates were added to the cells to a final concentration of 2 μM. Cells were incubated for 24 h in the presence of peptides for both assays. For the t-BHP control for the DCFH-DA assay, cells were incubated with 50 μM t-BHP for 4 h at 37 °C. The mixtures represent samples containing equimolar (1:1) WT and acetylated peptides. Error bars = ±SD (for A n = 5; and for B n = 4). *, **, and *** represent significant differences (* = p < 0.05), (** = p < 0.005), and (*** = p < 0.0005) among 7 d Aβ42 aggregated peptides.