(A–C) 8-AG decreases voiding frequency (A) (n = young, 13; aged, 21; aged + 8-AG, 30) and increases both the inter-void interval (B) (n = young, 13; aged, 22; aged + 8-AG, 30) and voided volume (C) (n = young, 13; aged, 21; aged + 8-AG, 22). (D and E) In addition, aging decreases abdominal (D) (n = young, 18; aged, 58; aged + 8-AG, 35) and cutaneous (E) (n = young, 13; aged, 21; aged + 8 AG, 23) responses to tactile mechanical stimuli, which is restored to a younger state by 8-AG treatment. Data are presented as mean ± SEM. Ordinary 1-way ANOVA was used to evaluate significance, which was considered at P < 0.05.