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. 2020 Oct 15;5(20):e140109. doi: 10.1172/jci.insight.140109

Figure 4. Western immunoblotting revealed significant aging-associated alterations in proteins linked to mitochondrial dynamics and quality control within the bladder mucosa.

Figure 4

(A–D) Mitofusin 2 (MFN2; n = young 14; aged 16; aged + 8-AG 16) (A), a protein involved in mitochondrial fusion; Dynamin-|related protein (B) (DRP-1; n = young 4; aged 5; aged + 8-AG 4), which is involved in mitochondrial fission; Parkin (C) (n = young 4; aged 5; aged + 8-AG 4), which plays a role in mitophagy; and cleaved caspase-3 (D) (n = young 8; aged 9; aged + 8-AG 8), which is activated upon initiation of apoptosis. In all cases, treatment with 8-aminoguanine (8-AG) restored changes similar to a younger state. Representative immunoblotting is inset within each graph. Cleaved caspase-3 (top of inset) is normalized to total caspase-3 (bottom of inset). All samples were run on the same blot, but representative samples were not contiguous. Data are presented as mean ± SEM. Ordinary 1-way ANOVA was used to evaluate significance. *P < 0.05; **P < 0.01.