Figure 5. Sequences from sorted naive cells support that T_N cells contribute to the HIV reservoir.

Sequences from sorted T_N cells were downloaded from Hiener et al. (14) and analyzed using our pipeline. The deletion maps were generated with the same bioinformatic tools used in Figure 3 and Figure 4 and Supplemental Figures 1 and 2 for our 2 subjects. No intact proviruses were detected for subject 2026, which might be explained by the low number of sorted T_N cells, as well as the long time the patient has been on ART. Nonetheless, the composition of the reservoir in T_N cells in these 3 individuals closely matches the proviral composition detected in T_N cells in Figure 3.