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. 2020 Oct 15;5(20):e139163. doi: 10.1172/jci.insight.139163

Figure 3. Immature neutrophils extravasate into temporal artery walls of GCA patient biopsies.

Figure 3

(A) Confocal image of a temporal artery section of a GCA biopsy stained for neutrophil Elastase (NE, red), CD15 (green), and Hoechst (gray) for DNA revealed presence of neutrophils in both the lumen and tissue. Scale bar: 200 μm. (B) Zoomed-in regions in a gallery overview exemplify the morphology of both segmented and unsegmented nuclei within the specimen and emphasize the individual staining (from left to right and top to bottom: all combined, Hoechst + CD15, Hoechst + NE, Hoechst-only nuclei). Arrowheads indicate unsegmented neutrophil nuclei. Scale bar: 20 μm. (C) The point map of the whole section shows categorization of neutrophils based on the nuclear shape. (D) The respective quantification of neutrophils on 1 section from 4 different biopsies.