Representative images of Masson’s trichrome–stained lung sections from 52-week-old WT (left, scale bar: 500 μm) and TRPV4–/– mice (right, scale bar: 200 μm) (A). Quantification of mean chord lengths of 4- to 6- (B), 28- to 30- (C), and 47- to 52-week-old (D) WT and TRPV4–/– mice. Inspiratory capacity (E), elastance of the respiratory system (F), static compliance (Cst, G) and compliance of the respiratory system (Crs, H) of 6-month-old WT and TRPV4–/– mice. Data represent mean ± SEM from at least 3 mice. Significance between means was analyzed using 2-tailed unpaired Student’s t test; *P < 0.05, **P < 0.01.