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. 2020 Oct 15;5(20):e139987. doi: 10.1172/jci.insight.139987

Figure 7. Effect of thalamic microinjection of Fgr siRNA on Coll IV microinjection–induced thalamic pain genesis.

Figure 7

(A–G) Effect of microinjection of Fgr siRNA (160 μM, 0.5 μL) or control scrambled siRNA (160 μM, 0.5 μL) into the VPL and VPM of the unilateral thalamus 4 days before Coll IV or saline microinjection into the same regions on paw withdrawal frequencies to 0.07 g (A and E) and 0.4 g (B and F) von Frey filaments and paw withdrawal latencies to heat (C and G) and cold (D) stimuli on the contralateral (A–D) and ipsilateral (E–G) sides on days 1, 3, and 5 after Coll IV/saline microinjection. n = 8 mice/group. Two-way ANOVA with repeated measures followed by post hoc Tukey’s test. **P < 0.01 versus the corresponding baseline (day –4). ##P < 0.01 versus the Coll IV plus vehicle group at the corresponding days. (H–J) Effect of microinjection of Fgr siRNA (160 μM, 0.5 μL) or control scrambled siRNA (160 μM, 0.5 μL) into unilateral thalamic VPL and VPM 4 days before Coll IV or saline microinjection into the same regions on the expression of Fgr, Lyn, and Src proteins in the ipsilateral (H and I) and contralateral (J) thalamus on day 5 post–Coll IV or saline microinjection. n = 3 biological repeats/group. Two-way ANOVA with repeated measures followed by post hoc Tukey’s test. *P < 0.05 versus the corresponding saline plus scrambled siRNA-treated group. #P < 0.05 versus the corresponding Coll IV plus scrambled siRNA-treated group.