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. 2020 Oct 15;5(20):e139987. doi: 10.1172/jci.insight.139987

Figure 8. Effect of thalamic microinjection of Fgr siRNA on Coll IV microinjection–induced thalamic activation of NF-κB and ERK1/2 pathways and production of TNF-α.

Figure 8

Expression of Iba1 and GFAP in the cytoplasmic fraction (A), expression of phosphorylated p65 (p-p65) and p-65 in the nuclear fraction (B), expression of phosphorylated ERK1/2 (p-ERK1/2) and ERK1/2 in the cytoplasmic fraction (C), and expression of p65 (total-p65) and TNF-α in the total cellular fraction (D) from the ipsilateral thalamus of the treatment groups on day 5 after thalamic microinjection of collagenase type IV (Coll IV) or saline. Top: representative Western blots. Bottom: statistical summary of the densitometric analysis. n = 3 biological repeats/group. Two-way ANOVA with repeated measures followed by post hoc Tukey’s test. **P < 0.01 versus the corresponding saline plus scrambled siRNA-treated group. #P < 0.05, ##P < 0.01 versus the corresponding Coll IV plus scrambled siRNA-treated group.