Figure 3. Absence of the Pad2 gene improves bacterial clearance in a CLP murine model of sepsis and enhances phagocytosis in BM-derived macrophages (BMDMs).

WT and Pad2^–/– mice were subjected to CLP. (A) Bacterial loads in peritoneal lavage, blood, and spleen (n = 4–5/group) after 24 hours. BMDMs were isolated from WT and Pad2^–/– mice and incubated with 1 mg/mL pHrodo Red E. coli BioParticles. (B and C) Fluorescence signal of engulfed E. coli bioparticles in peritoneal macrophage (B) and its representative images (C). Values are relative fluorescence units (RFU), calculated from 3 wells per group. The results in B and C are representative of 3 independent experiments. Data are expressed as mean ± SEM. Data with 2 groups were analyzed using an unpaired 2-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001. White scale bar: 20 μm. Red scale bar: 10 μm.