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[Preprint]. 2020 Oct 30:2020.10.23.347534. [Version 2] doi: 10.1101/2020.10.23.347534

Figure 3. K777 alkyne specifically targets a non-viral protein in SARS-CoV-2 infected and uninfected Vero E6 cells.

Figure 3.

A. Cy7 azide labeled proteins (SARS-CoV-2 infected and uninfected) at 1 μM K777 alkyne is blocked by pre-treatment with 1 μM K777. B. Densitometry analysis of A, and a replicate in gel fluorescence experiment. C. Blotting of total β-actin levels in SARS-CoV-2 infected and non-infected cells. D. Densitometry analysis of relative actin levels. E. Comparison of the signal intensity of the 25 kDa band enriched in the presence of K777 alkyne (SARS-CoV-2 infected and uninfected) versus the β-actin signal (SARS-CoV-2 infected and unifected).