Figure 3. Replication and transmission of SARS-CoV-2^S−614D and SARS-CoV-2^S−614G viruses in Syrian hamsters.

Transmission of SARS-CoV-2^S−614D and ^S−614G variant by hamsters in a pairwise one-by-one setup with direct contact of donor and cohoused contact hamsters is illustrated. Samples of nasal washings were taken daily between days 2 to 8 post infection (dpi) and finally at 12 dpi and were analyzed. Pie chart representation of fraction of A or G nucleotide at position 23,403 corresponding to SARS-CoV-2^S−614D and SARS-CoV-2^S−614G, respectively, measured by amplicon sequencing. Genome copies were calculated from RT-qPCR using a standard RNA. Orange coloring of the hamster silhouette refer to detection of G (SARS-CoV-2^S−614G), while blue coloring indicates detection of A (SARS-CoV-2^S−614D) on most time points. Grey coloring signals no infection detected