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. 2020 Oct 29;12:10735–10747. doi: 10.2147/CMAR.S263538

Figure 6.

Figure 6

MALAT1 targeted miR-613 to regulate the expression of COMMD8. (A) The binding sites between MALAT1 and miR-613 were forecasted by the online tool starbase. (B and C) The relationship between MALAT1 and COMMD8 was verified by dual-luciferase reporter assay. (D) COMMD8 was predicted to be a target of miR-613 with a specific binding site at its 3ʹUTR. (E and F) The relationship between miR-613 and COMMD8 was confirmed by dual-luciferase reporter assay. (G) The expression of miR-613 in A549 and H1299 cells transfected with si-MALAT1 or si-NC was detected by qRT-PCR. (H) The expression of miR-613 in A549 and H1299 cells transfected with miR-613 inhibitor or inhibitor-NC was detected by qRT-PCR. (I–K) The expression of COMMD8 in A549 and H1299 cells transfected with miR-613 inhibitor or inhibitor-NC was examined by qRT-PCR and Western blot at both mRNA and protein levels. (L and M) The expression of COMMD8 in A549 and H1299 cells transfected with si-MALAT1, si-NC, si-MALAT1+miR-613 inhibitor or si-MALAT1+inhibitor-NC was examined by qRT-PCR and Western blot at both mRNA and protein levels. *P < 0.05.