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. 2020 Oct 29;15:8465–8478. doi: 10.2147/IJN.S272571

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© 2020 Zhou et al.

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Osteogenic differentiation analysis.

Notes: (A) ALP staining of cells on MBG/SIS, MBG/SIS-P28, and MBG/SIS-H-P28 scaffolds at 7 and 14 days. (B) The quantification of ALP activity in cells on scaffolds at 7 and 14 days. (C) The ARS staining, a marker for minerals deposited by cells on scaffolds at 14 and 21 days. (D) The quantification of minerals deposited by cells on scaffolds at 14 and 21 days. Statistical significance is indicated by *p < 0.05 compared with MBG/SIS group and ^#p<0.05 compared with MBG/SIS-P28.

Abbreviations: MBG, mesoporous bioactive glass; SIS, small intestinal submucosa; ALP, alkaline phosphatase; ARS, Alizarin Red S.