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| Primer | Forward | Reverse |
|---|---|---|
| GAPDH | F5ʹ-GACAAAATGGTGAAGGTCGGT | R5ʹ-GAGGTCAATGAAGGGGTCG |
| Runx2 | F5ʹ-AACTTGCTAACGTGAATGGTC | R5ʹ-TAGCCCACTGAAGAAACTTGG |
| Opn | F5ʹ-GAGGTGATAGCTTGGCTTACGG | R5ʹ-ACGCTGGGCAACTGGGAT |
| Ocn | F5ʹ-GAACAGACAAGTCCCACACAG | R5ʹ-TCAGCAGAGTGAGCAGAAAGAT |
| ALP | F5ʹ-ACCACCACGAGAGTGAACCA | R5ʹ-CGTTGTCTGAGTACCAGTCCC |
