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. 2020 Oct 29;13:11059–11070. doi: 10.2147/OTT.S235643

Figure 2.

Figure 2

DUXAP8 increased CRC cell proliferation and cell cycle progression, and inhibited apoptosis. (A, B) Proliferating SW620 and LoVo cells were labeled with Edu. The click-it reaction revealed Edu staining (red). Cell nuclei were stained with DAPI (blue). (C, D) CCK8 assays revealed that DUXAP8 silencing inhibited SW620 cell proliferation, while overexpression DUXAP8 promoted LoVo cell proliferation. (E) Flow cytometry was used to detect the apoptotic rates of cells. UR, early apoptotic cells; LR, terminal apoptotic cells. (F) Flow cytometry was used to detect the cell cycle distribution of cells. Silencing of DUXAP8 increased the percentage of SW620 cells in G0/G1 phase, while overexpression of DUXAP8 reduced the percentage of LoVo cells in G0/G1 phase. (G) The apoptosis ratio as affected by DUXAP8 silencing and DUXAP8 overexpression. (H) The cell cycle distribution as affected by DUXAP8 silencing and DUXAP8 overexpression. *P < 0.05, **P < 0.01, ***P < 0.001 vs NC; ##P < 0.01, ###P < 0.001 vs Vector.