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. 2020 Oct 29;13:11125–11137. doi: 10.2147/OTT.S273108

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© 2020 Gan et al.

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Gene expression level in un-treated NB cells and the cell viability of NB cells treated with 3-BrPA of different concentration. (A) The mRNA expression level of N-Myc, C-Myc, P53, MCT1 and SQSTM1 in NB cell lines (SK-N-BE2, IMR-32, SK-N-AS, SH-SY5Y and SK-N-SH) by QPCR analysis. (B) Metabolism related gene expression (HK2, LDHA, PDK and PKM) in NB cell lines by QPCR analysis. (C) The protein expression level of N-Myc, C-Myc, MCT1, SQSTM1 and LC3 (LC3-II/LC3-I) in NB cell lines by WB analysis. (D) The cell proliferation ability of 3-BrPA treated NB cells in different concentration by CCK-8 analysis (*P<0.05; **P<0.01; ***P<0.001).