Fig 4. Detection of Nef in neurons from hippocampus of SIV- infected macaques.
A. Hippocampus regions from paraffin embedded SIV-infected macaques brain samples were serial sectioned and stained for Neu-N (pink) and Nef (green) using opal multiplex immunohistochemical assay staining. Representative images were taken at 20X magnification with a Keyence BZ-X700 microscope. (n = 3/ per group). B. Quantification analysis of number of Nef+ within NeuN + cells in hippocampus region per mm2, five images were taken per condition per tissue and the average number per group was used (n = 3). Comparisons of means for significance was determined with T-test. Scale bar represent 10 μM. (P < 0.002).