Fig 4. Androgen promotes LPCAT1 nuclear relocalization.

(A): Intra-nuclear LPCAT1 expression in LPCAT1 and control groups and cells were cultured with ordinary FBS containing a certain amount of androgen. (B): The effect of the increased concentrations of DHT on intra-nuclear LPCAT1 expression of C4-2 cells cultured in Dextran Stripped FBS was detected. (C): The effect of DHT (10⁻⁶ M), DHT (10⁻⁶ M) +Flu and DHT (0 M) on the proliferation ability of C4-2 cells cultured in Dextran Stripped FBS was detected. (D): Tumors weights in 6 nude mice with LPCAT1 and control groups after castration. (E): Activated RNA polymerase (RNAP) Ⅱ expression (up) and total RNA synthesis (down) were measured in LPCAT1 and control groups cells with ordinary FBS culture. (F): Histone H4 S47A mutation was designed and transfected into LPCAT1 and control groups, and activated RNA polymerase (RNAP) Ⅱ expression (up) and total RNA synthesis (down) were measured in WT and mutation groups. Values are presented as mean percent control ± s.e.m or mean ± s.e.m. * P < 0.05 and ** P < 0.01 compared with control. # P < 0.05 and ## P < 0.01 compared with LPCAT1 overexpressing cells in WT group.