Table 2.

Sequences of primers and probes. Sequence of forward (F) and reverse (R) primers and of dual-labeled probes are reported. Expected amplicon size and cycling parameter are given, as per Chu et al. (2020).

Organism	Target gene	Assay name	Sequence (5′-3′)	Amplicon size	Cycling parameters	Reference
SARS-CoV-2	Orf1b	HKU-ORF1b-nsp14F	F-TGGGGYTTTACRGGTAACCT	132 bp		Chu et al., 2020
		HKU-ORF1b-nsp14R	R-AACRCGCTTAACAAAGCACTC		50 °C for 5 min;
		HKU-ORF1b-nsp141P	P-FAM-TAGTTGTGATGCWATCATGACTAG-QSY		95 °C for 20 s;
	N	HKU-NF	F-TAATCAGACAAGGAACTGATTA	110 bp	40 cycles of 95 °C for 5 s;
		HKU-NR	R-CGAAGGTGTGACTTCCATG		60 °C for 30 s.
		HKU-NP	P-FAM-GCAAATTGTGCAATTTGCGG-QSY