Figure 1. Schematic, Dissolved Oxygen, and Growth Profiles of the Controlled O₂ Model Reactor System.

(A) Schematic of controlled O₂ model reactor system. Four reactors were multiplexed and individually monitored for DO levels to obtain biological replicates.

(B) DO levels across the 120 h time course.

(B and C) Points are the average of three biological replicates and error bars represent standard deviation; the yellow shading indicates the periods of controlled O₂ depletion and reaeration, whereas the gray background indicates a sustained 2 day immersion in hypoxia.

(C) Growth of H37Rv in fully aerated cultures (triangles, dashed line) or cultures from the controlled O₂ model reactor system (circles, solid line). Growth was monitored daily (aerated cultures) or at each sampling time points (controlled O₂ model cultures) by optical density at 650 nm.