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. 2020 Nov 2;9:e56059. doi: 10.7554/eLife.56059

Figure 1. Expression of corin and other selected proteases in MDCK, HL-1, and HEK293 cells.

(A) MDCK (left), HL-1 (middle), and HEK293 (right) cells were transfected with a plasmid expressing corin. After 72 hr, immunostaining was done to examine corin (green) and ZO-1 (red) (an indicator of the apicolateral tight junction) expression with confocal microscopy. X-Y and X-Z views are shown in top and lower panels, respectively. (B) Neprilysin, hepsin, matriptase, and TMPRSS3-5 (green) were expressed in transfected MDCK cells. Immunostaining and confocal microscopy were used to examine protein expression on apical and basolateral membranes. X-Y and X-Z views are shown in top and lower panels, respectively. Each image represents the data from five experiments. Scale bars: 5 μm.

Figure 1.

Figure 1—figure supplement 1. Western blotting analysis of corin expression in HEK293 and MDCK cells.

Figure 1—figure supplement 1.

Corin modules, including the cytoplasmic tail, the transmembrane domain (TM), frizzled (Fz), LDLR, scavenger receptor (SR), and protease domain modules, are shown. The activation cleavage site is indicated by an arrow. Recombinant corin had a C-terminal V5 (V) tag for protein detection. Western blotting (under reducing conditions) was done with an anti-V5 antibody to analyze corin protein fragments in transfected MDCK cells without (-) or with (+) trypsin treatment before the cells being lysed. Corin zymogen and cleaved protease domain fragments are indicated. The data are representative of four experiments.