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. 2020 Nov 2;9:e56059. doi: 10.7554/eLife.56059

Figure 6. Expression of human CD320 in kidney sections and MDCK cells.

(A) Human CD320 protein contains an N-terminal extracellular region with two LDLR modules and one EGF module, a transmembrane (TM) domain, and a C-terminal cytoplasmic tail. LDLR1 module has a DGSDE motif, whereas LDLR2 module has a DSSDE motif (underlined). (B) Co-staining of neprilysin (brush border marker) (green), corin (red or green) and CD320 (red) in proximal tubules in human kidney sections. Cell nuclei were stained in blue. Boxed areas in top panels are enlarged in lower panels. Scale bars: 10 μm. (C) Corin (green), CD320 (green), and ZO-1 (red) expression in transfected MDCK cells. X-Y and X-Z views are shown in top and lower panels, respectively. Scale bars: 5 μm. Each image represents data from five experiments.

Figure 6.

Figure 6—figure supplement 1. Structural alignments of the LDLR domains of human CD320.

Figure 6—figure supplement 1.

(A) The structure alignments of human CD320 LDLR1 (residues 54–89) (light blue) and LDLR2 (residues 132–166) (violet), and corin LDLR8 module (residues 655–689) (cyan) with the calcium ions (spheres) and side chains and disulfide bonds (sticks). (B) The DSSDE motifs in CD320 LDLR2 and corin LDLR8 modules are enlarged from (A). (C) The local alignment of the DSSDE motifs in CD320 LDLR2 and corin LDLR8 modules.