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. 2020 Nov 2;9:e56059. doi: 10.7554/eLife.56059

Figure 7. Expression of CD320 mutants with point mutations in the DSSDE motif in LDLR2 module.

(A) Human CD320 WT and mutants with point mutations in LDLR2 module at indicated positions shown in red (D160A, S161A, S162A, D163A, and E164A) were expressed in MDCK cells. CD320 expression (green) on apical and basolateral membranes was examined by immunostaining and confocal microscopy with X-Y (top panels) and X-Z (lower panels) views. ZO-1 protein (red) was used as an indicator. (B) CD320 WT and S162A mutants without (S162A) or with the DSSDE motif created in LDLR1 module (ML1/S162A) expressed in MDCK cells were examined by immunostaining and confocal microscopy. X-Y (top panels) and X-Z (lower panels) views are indicated. Each image represents the data from five experiments. Scale bars: 5 μm.

Figure 7.

Figure 7—figure supplement 1. Quantitative analysis of CD320 and corin expression in MDCK cells.

Figure 7—figure supplement 1.

(A and B) CD320 WT and mutant expression in MDCK cells was examined by immunostaining and confocal microscopy. Fluorescent signals along the X-Z axis were analyzed with Image J software to calculate the ratio of FBL/FTotal. The data are mean ± SEM (n = 5).
Figure 7—figure supplement 1—source data 1. Source data for Figure 7—figure supplement 1A.
Figure 7—figure supplement 1—source data 2. Source data for Figure 7—figure supplement 1B.