Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (H. sapiens) |
CORIN | NCBI | NM_006587.4 | |
| Gene (H. sapiens) |
ST14 (matriptase) | NCBI | NM_021978.4 | |
| Gene (H. sapiens) |
TMPRSS3 | NCBI | NM_032404.3 | |
| Gene (H. sapiens) |
TMPRSS4 | NCBI | NM_019894.4 | |
| Gene (H. sapiens) |
TMPRSS5 | NCBI | NM_030770.4 | |
| Gene (H. sapiens) |
MME (neprilysin) | NCBI | NM_007287.4 | |
| Gene (H. sapiens) |
CD320 | NCBI | NM_016579.4 | |
| Cell line (H. sapiens) |
HEK293 | ATCC | CRL-1573 | STR profiling, no mycoplasma contamination |
| Cell line (Canis familiaris) |
MDCK | CCTCC* | 3142C0001000000147 | STR profiling, no mycoplasma contamination |
| Cell line (Mus musculus) |
HL-1 | Sigma-Aldrich | SCC065 (Originally from William Claycomb) |
No mycoplasma contamination |
| Transfected construct (H. sapiens) |
Corin plasmid | PMID:14559895 | pcDNA 3.1/V5-His | |
| Transfected construct (H. sapiens) |
Neprilysin plasmid | PMID:27343265 | pCMV3-C-Flag | |
| Transfected construct (H. sapiens) |
Hepsin plasmid | PMID:31395734 | pcDNA 3.1/V5-His | |
| Transfected construct (H. sapiens) |
Matriptase plasmid | This paper | pcDNA 3.1/V5-His | |
| Transfected construct (H. sapiens) |
TMPRSS3 | This paper | pcDNA 3.1/V5-His | |
| Transfected construct (H. sapiens) |
TMPRSS4 | This paper | pcDNA 3.1/V5-His | |
| Transfected construct (H. sapiens) |
TMPRSS5 | This paper | pcDNA 3.1/V5-His | |
| Transfected construct (H. sapiens) |
CD320 | This paper | pcDNA 3.1/N-Flag | |
| Transfected construct (Canis familiaris) |
Rab11a | This paper | pCMV3-C-OFPSpark | |
| Transfected construct (Canis familiaris) |
Rab11b | This paper | pCMV3-C-OFPSpark | |
| Transfected construct (H. sapiens) |
Corin mutants | This paper | pcDNA 3.1/V5-His | |
| Transfected construct (H. sapiens) |
CD320 mutants | This paper | pcDNA 3.1/N-Flag | |
| Antibody | Anti-corin (rabbit polyclonal) |
PMID:27343265 | 1:1000 | |
| Antibody | Anti-corin (mouse monoclonal) |
PMID:22437503 | 1:1000 | |
| Antibody | Anti-CD320 (rabbit polyclonal) |
Atlas Antibodies | HPA073489 | 1:10 |
| Antibody | Anti-neprilysin (mouse monoclonal) |
Boster | BM3411 | 1:100 |
| Antibody | Anti-Flag (mouse monoclonal) |
Sigma | F1804 | 1:500 |
| Antibody | Anti-V5 (mouse monoclonal) |
Thermo Fisher | R96025 | 1:500 |
| Antibody | Anti-V5-HRP (mouse monoclonal) |
Thermo Fisher | R96125 | 1:5000 |
| Antibody | Anti-ZO-1 (rabbit polyclonal) |
Cell Signaling | 8193 s | 1:300 |
| Antibody | Anti-IgG-Alexa-488 (donkey polyclonal) |
Thermo Fisher | A32766 | 1:500 |
| Antibody | Anti-IgG-Alexa-594 (goat polyclonal) |
Thermo Fisher | A32740 | 1:500 |
*CCTCC: China Center for Type Culture Collection.