Table 2.
Primers Used for Plasmid Construction and for RT-qPCR
| Primer Name | Sequence (5 ‘–3 ‘) |
|---|---|
| HBV-miR-3 RT | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACAGAAGAAC |
| HBV-miR-3 Forward | TGCGGATCTTCTTGTTGGTT |
| HBV-miR-3 prob | FAM- CACTGGATACGACAAACGCCGCAGA –BHQ1 |
| U6 RT | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACAAAATATGG |
| U6 Forward | TGCGGGTGCTCGCTTCGGCAGC |
| U6_prob | Hex- CACTGGATACGACAAAATATGGAAC –BHQ1 |
| miRNA Reverse prim | CCAGTGCAGGGTCCGAGGT |
| HBV-miR-3 agomir | CUGGAUGUGUCUGCGGCGUUU |
| HBV-miR-3 antagomir | AAACGCCGCAGACACAUCCAG |
| HBV-miR-3 NC | CAGUACUUUUGUGUAGUACAA |
| PTEN-3UTR-miR3BS-F | CTCTTGTTTTACCCTATACATCCACAGGGTTTTGACACTTGT |
| PTEN-3UTR-miR3BS-R | CTAGACAAGTGTCAAAACCCTGTGGATGTATAGGGTAAAACAAGAGAGCT |
| PTEN-3UTR-miR3BSmut-F | CTCTTGTTTTACCCTATCTGGTGTCAGGGTTTTGACACTTGT |
| PTEN-3UTR-miR3BSmut-R | CTAGACAAGTGTCAAAACCCTGACACCAGATAGGGTAAAACAAGAGAGCT |