Figure 3.
Downregulation of HOXB7 enhances sensitivity to cisplatin. (a) We established stable HOXB7‐knockdown cell strains (KYSE450‐HOXB7i, KYSE150‐HOXB7i) to determine the effect of HOXB7 on chemosensitivity. CCK8 assay showed that cell viability of HOXB7‐knockdown cells was reduced with a smaller IC50 value than control cells (1.384 vs. 15.170 for KYSE150, 1.591 vs. 5.703 for KYSE450). (
) Cis and (
) HOXB7i‐Cis (b) Colony formation assay showed that there were less colony formation in HOXB7‐knockdown cells upon exposure to cisplatin (2 μg/mL for KYSE150 and 1 μg/mL for KYSE450) than control cells. (
) Control, (
) Cis, (
) HOXB7i, and (
) HOXB7i‐Cis. (c) HOXB7‐knockdown sensitizes ESCC cells to cisplatin (2 μg/mL for KYSE150 and 1 μg/mL for KYSE450) induced apoptosis. All experiments were performed thrice in triplicates. (
) Control, (
) Cis, (
) HOXB7i, and (
) HOXB7i‐Cis.