Figure 5.
AKT/MDM2/p53 signaling pathway participates in the protective effect of HAX1 in lung cancer. (a) Phosphorylation of MDM2 was reduced and p53 levels in A549 and H1299 cells transfected with si‐HAX1. (b) Quantitative analysis of protein p‐MDM2 and p53 expression in A549 and H1299 cells. (
) A549/si‐NC, (
) H1299/si‐NC, (
) A549/si‐HAX1 and (
) H1299/si‐HAX1. (
) A549/si‐NC, (
) H1299/si‐NC, (
) A549/si‐HAX1 and (
) H1299/si‐HAX1.(c) SC79 promoted the phosphorylation of MDM2 and reduced p53 level in A549 and H1299 cells transfected with si‐HAX1. (d) Quantitative analysis of protein p‐MDM2, and p53 expression in A549 and H1299 cells. ***P < 0.001, **P < 0.01, *P < 0.05.