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. 2020 Sep 15;10(10):1835–1845. doi: 10.1016/j.apsb.2020.09.005

Table 1.

Correlated MS and proteomics data matching coagulopathic venom toxins.

Species Peak retention time (min) Peak activity Mascot results matching the exact mass Exact mass from MS data Exact mass calculated from Mascot data Toxin class Dose required for full inhibition
B. asper 18.9–20.0 Anticoagulation PA2H2_BOTAS 13,714.565 13,715 PLA2 PN
20.1–21.0 Anticoagulation PA2HA_BOTAS 13,912.465 13,897 PLA2 PN
20.1–21.0 Anticoagulation PA2H3_BOTAS 13,765.581 13,766 PLA2 PN
21.0–21.5 Anticoagulation PA2B3_BOTAS 13,957.533 13,957 PLA2 PN
21.0–21.5 Anticoagulation PA2A2_BOTAS 14,194 PLA2 PN
21.0–21.5 Anticoagulation VM2_BOTAS 53,564 SVMP NOI
20.8–22.4 Procoagulation VSPL_BOTAS 28,019 SVSP PN
20.8–22.4 Procoagulation VM1B1_BOTAS 45,936 SVMP PN
21.2–22.4 Procoagulation SLA_BOTAS 7084 CTL PN
B. jararaca 20.5–21.1 Anticoagulation NOI
20.2–21.7 Procoagulation VSPA_BOTJA 25,584 SVSP PN
20.2–21.7 Procoagulation VSP1_BOTJA 25,742 SVSP PN
20.2–21.7 Procoagulation VSP2_BOTJA 27,894 SVSP PN
20.2–21.7 Procoagulation VSP12_BOTJA 27,988 SVSP PN
20.2–21.7 Procoagulation VSP14_BOTJA 27,843 SVSP PN
20.2–21.7 Procoagulation VSP20_BOTJA 27,815 SVSP PN
C. rhodostoma 19.3–20.5 Anticoagulation PA2BD_CALRH 13,665.085 13,665 PLA2 NOI
19.3–20.5 Anticoagulation PA2AB_CALRH 14,352 PLA2 NOI
19.3–20.5 Anticoagulation VSPF1_CALRH 26,570 SVSP NOI
19.3–20.5 Anticoagulation SLEA_CALRH 15,962 CTL NOI
19.3–20.5 Anticoagulation SLEB_CALRH 15,190 CTL NOI
19.3–21.6 Procoagulation VSPF2_CALRH 29,145 SVSP PN
19.9–21.6 Procoagulation SLYA_CALRH 15,796 CTL PN
19.9–21.6 Procoagulation SLYB_CALRH 16,770 CTL PN
D. acutus 20.4–20.9 Anticoagulation PA2A_DEIAC 14,820 PLA2 4 μmol/L varespladib
20.4–20.9 Anticoagulation SL_DEIAC 18,332 CTL 4 μmol/L varespladib
21.4–21.7 Anticoagulation NOI
21.0–22.7 Procoagulation VSP1_DEIAC 29,480 SVSP PN
21.0–22.7 Procoagulation VSPA_DEIAC 26,132 SVSP PN
21.4–22.7 Procoagulation SLCB_DEIAC 17,133 CTL PN
21.4–22.7 Procoagulation VM1AC_DEIAC 47,690 SVMP PN
21.4–22.7 Procoagulation VM11_DEIAC 47,845 SVMP PN
21.4–22.7 Procoagulation VM1H5_DEIAC 46,518 SVMP PN
21.4–22.7 Procoagulation VM3AK_DEIAC 69,752 SVMP PN
22.7–23.4 Procoagulation VM3A2_DEIAC 27,151 SVMP 20 μmol/L varespladib/4 μmol/L marimastat
22.7–23.4 Procoagulation VM3AH_DEIAC 70,721 SVMP 20 μmol/L varespladib/4 μmol/L marimastat

Peak retention times are adapted from Figure 1, Figure 2, Figure 3, Figure 4; PLA2 = phospholipase A2; SVMP = snake venom metalloproteinase; SVSP = snake venom serine protease; CTL=C-type lectin; PN = partly neutralised at 20 μmol/L inhibitor concentrations; NOI = no observed inhibition; ‒ Not applicable.