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. 2020 Feb 27;10(10):1943–1953. doi: 10.1016/j.apsb.2020.02.010

Figure 3.

Figure 3

The degradation of IDO1 by PROTAC degraders. (A) Degradation of IDO1 by 2c under different concentration of IFN-γ. HeLa cells were treated with 2c and IFN-γ for 24 h. (B) IFN-γ upregulate the expression of cellular IDO1 mRNA. Data are expressed as the mean ± SD (n = 3), error was shown as shaded area. (C) Capacity of degradation of degraders. 10 μmol/L of degraders and 5 ng/mL of IFN-γ were incubated with HeLa cells for 24 h. As determined by Western blot, almost complete degradation of IDO1 was observed in 2c. (D) Determination of degradative ability of 2c. Compound 2c and IFN-γ (5 ng/mL) were incubated with HeLa cells for 24 h, and a significant dose-dependent degradation was observed. (E) The determination of degradative ability of 2c by immunoblot band intensity. Data are expressed as the mean ± SD (n = 3).