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. 2019 Jun 26;10(10):1977–1988. doi: 10.1016/j.apsb.2019.06.008

Figure 3.

Figure 3

(A) The colocalization of GLT and lysosomes. GLT was labeled with DiD (red), lysosomes were stained with Lyso-Tracker Red (green), and cell nucleuses were stained with DAPI (blue). (B) Cellular uptake of GT and GLT in 4T1 cells (means ± SD, n = 3; ∗P＜0.05,∗∗P＜0.01 and ∗∗∗P＜0.001). (C) Growth inhibitory effect of GEM and GLT on 4T1 cells. (D) Growth inhibitory effect of LT on 4T1 cells (means ± SD, n = 3; ∗P＜0.05,∗∗P＜0.01 and ∗∗∗P＜0.001) (E) Images of scratch after incubation with free GEM, LMWH and GLT, respectively, for 12 h. (F) Scratch healing rates calculated by Image J (means ± SD, n = 3; ∗P＜0.05,∗∗P＜0.01 and ∗∗∗P＜0.001). (G) The fluorescence intensity of platelets adhering to 4T1 cells detected by a Varioskan Flash Multimode Reader (Thermo, USA). “+” indicates coincubation with calcein-AM labeled platelets, “–” indicates no coincubation with calcein-AM labeled platelets (means ± SD, n = 3; ∗P＜0.05,∗∗P＜0.01 and ∗∗∗P＜0.001). (H) Expression of MMP-9 in 4T1 cells tested by Western blot after separate incubation with PBS, LMWH (60 μg/mL), LT (100 μg/mL) and LT (250 μg/mL).