Fig. 5.

Effects of c-Met on cell migration and invasion in colon cancer cells

c-Met enhanced cell migration and invasion in colon cancer cells. Silencing c-Met expression via c-Met-specific siRNA reversed the migration and invasion of colon cancer cells. Colon cancer cells DLD1 (a), RKO (b), and CT26 (c) were transfected with either small interfering RNA (siRNA)-negative control (NC) or siRNA-c-Met and incubated for 24 h. The silencing efficiency is confirmed by western blot assay. Another group of silencing cells was treated with verticillin A (VA) for 24 h. Then, Transwell assay was used to detect the trans-membrane migration ability of these cells. Densitometric analysis of the protein bands was done using ImageJ (National Institutes of Health (NIH)). Magnification: 10×10. Data are presented as mean±standard deviation (SD), n=3. ^** P<0.01