Fig. 6.

Effect of verticillin A on c-MET phosphorylation

Western blot analysis showed that verticillin A (VA) inhibited c-MET phosphorylation activated by hepatocyte growth factor (HGF) in colon cancer cells. DLD1 (a), RKO (b), and CT26 (c) were treated with 40 ng/mL HGF or co-treated with 40 ng/mL HGF and VA for 0, 3, 6, and 9 h, and then proteins were extracted. Western blot analysis showed the expression of c-MET and p-MET, and β-actin was used as loading control. Densitometric analysis of the protein bands was done using ImageJ (National Institutes of Health (NIH)). Data are presented as mean±standard deviation (SD), n=3. ^* P<0.05, ^** P<0.01, vs. HGF