Fig. 8.

Effect of verticillin A on the activation of c-MET downstream molecules

Verticillin A suppressed the activation of c-MET downstream molecules. Western blot analysis showed protein levels in downstream signaling of c-MET regulated by verticillin A. Colon cancer cells DLD1, RKO, and CT26 were treated with different concentrations of verticillin A for 48 h and then proteins were extracted. Western blot analyzed the key protein levels that were regulated downstream of c-MET signaling, and β-actin was used as loading control. Densitometric analysis of the protein bands was done using ImageJ (National Institutes of Health (NIH)). Data are presented as mean±standard deviation (SD), n=3. ^* P<0.05, ^** P<0.01, vs. control (0 μmol/L verticillin A)