Fig. 9.

Effect of Erk on the migration–inhibition of verticillin A

Overexpression of Erk partially reversed the migration–inhibition of verticillin A. (a) DLD1 and RKO cells were transiently transfected with an Erk-expressing construct or an empty vector for 24 h. Cells were analyzed by western blot for the expression level of ERK. The protein levels were quantified and presented in the bottom panels. (b) The transfected colon cancer cells were treated with verticillin A at 400 nmol/L (for DLD1 cells) or 200 nmol/L (for RKO cells) for 24 h, and analyzed by western blot for ERK and p-ERK protein levels. The protein levels were quantified and presented in the bottom panels. (c) The transfected colon cancer cells were seeded onto the upper chamber and treated with verticillin A for 24 h. Cell migratory behaviors were analyzed by migration chamber assay. The relative quantitative determination of migrated cells was calculated with five fields counted per experiment. Magnification: 10×10. Data are presented as mean±standard deviation (SD) from three independent experiments. ^* P<0.05, ^** P<0.01