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内源性表达FGFR2-CCDC6融合蛋白的异种移植物(LIV31)的构建与验证

Establishment and verification of a xenograft (LIV31) endogenously expressing FGFR2-CCDC6 fusion protein. A: Abdominal contrast-enhanced CT image of the patient before surgery. B: Chest contrast-enhanced CT image after resection (9 months later). C-D: HE staining of primary carcinoma tissue and lung metastatic tumor. E: Sanger sequencing results of RT-PCR products. F: LIV31 grows subcutaneously on the flanks of nude mice. G: Tumor tissue excised from LIV31 xenograft in nude mice. H-K: FGFR2 break-apart FISH results from control tissues, primary liver tumors, lung metastases and xenografts. L: G-banded karyogram of the LIV31 cell line. M: Metaphase state of LIV31 cell line using FGFR2 BAC and D10Z1 probes. N: Metaphase spread of LIV31 cell line with the application of whole chromosome paint 10 probe.