FIGURE 6.

Sequence alignment of NisP from Lactococcus lactis and NshP from Streptococcus hyointestinalis. (A) The important residues from the active site (Asp₂₅₉, His₃₀₆, and Ser₅₁₂ for NisP and Asp₁₇₈, His₂₂₅, and Ser₄₃₁ for NshP) are marked in red, shown together with the close neighborhood residues. The sequence alignment was done with Clustal Omega (Madeira et al., 2019). (B) Zoom-in into the active sites of NshP (magenta) and NisP (cyan). The important residues for the catalytic activity (Asp₂₅₉, His₃₀₆, and Ser₅₁₂ for NisP and Asp₁₇₈, His₂₂₅, and Ser₄₃₁ for NshP) are shown in stick representation. Figure was generated using Pymol (2015). For a full alignment and a corresponding homology model, please see Supplementary Material.