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. 2020 Oct 20;11:578176. doi: 10.3389/fimmu.2020.578176

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Copyright © 2020 Gonder, Fernandez Botana, Wierz, Pagano, Gargiulo, Cosma, Moussay, Paggetti and Largeot

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of the contamination due to metal oxidation during the titration. (A) The samples are concatenated in order to display the 5 dilutions for the CD45RA antibody in the same graph (1 to 5: range from 1:1 to 1:81 dilutions) (B) Using the second dilution (1:3), the CD45RA antibody gives a signal in the empty 159Tb channel, corresponding to oxidation of the metal (red box). (C) This signal is clearly reduced using the 5^th dilution (1:81). This dilution will be used as it has the lowest contamination, still giving a good positive signal over negative one (Blue and green boxes).