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. 2020 Oct 20;11:578176. doi: 10.3389/fimmu.2020.578176

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Copyright © 2020 Gonder, Fernandez Botana, Wierz, Pagano, Gargiulo, Cosma, Moussay, Paggetti and Largeot

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Example of 6-choose-3 barcoding matrix with 6 palladium isotypes. Heavy metal-labeled cellular barcoding enables running multiple samples in one tube by staining each sample with a unique combination of isotopes before pooling the samples. Here, up to 20 samples can be combined by different staining pattern. Following oftware debarcoding, samples can be analyzed individually. For example, sample 1 will be positive for Palladium 102, 104, 105, but negative for the 106, 108, and 110 isotopes. Adapted from Fluidigm.